胶原蛋白酶2,9检测试剂盒(MMP Zymograhpy A ssay Kit)
基质金属蛋白酶(matrix metalloproteinase, MMP)以无活性的酶原形式分泌,活化后能降解细胞外基质的胶原蛋白,又称胶原酶。胶原酶通过影响细胞外基质参与胚胎发育、形态发生、组织重塑等过程,并参与炎症、肿瘤、心血管、神经等许多疾病过程。血浆和组织中的MMP-2、MMP-9参与各种生理与病理过程,其在临床诊断和治疗中的意义日益受到重视。本试剂盒采用酶谱法(zymography)检测MMP-2、MMP-9活性。Zymography是一种广为使用的、基于SDS-PAGE电泳和反相凝胶染色的蛋白酶检测方法,其灵敏度可达1 nM,高于ELISA方法。
部分使用胶原蛋白酶2,9检测试剂盒(P1700)发表的文章,供参考。
1、 Zi Y, Zhao W, Zhou J, et al. Silencing of TMSG1 enhances metastasis capacity by targeting V-ATPase in breast cancer[J]. International journal of clinical and experimental pathology, 2015, 8(2): 1312.
2、 Zhang L, Liu C, Meng X, et al. Smad2 protects against TGF-β1/Smad3-mediated collagen synthesis in human hepatic stellate cells during hepatic fibrosis[J]. Molecular and cellular biochemistry, 2015: 1-12.
3、 Wang S H, Zhou J D, He Q Y, et al. MiR-199a inhibits the ability of proliferation and migration by regulating CD44-Ezrin signaling in cutaneous squamous cell carcinoma cells[J]. International journal of clinical and experimental pathology, 2014, 7(10): 7131.
4、 Wang H, Zhu Y, Zhao M, et al. miRNA-29c suppresses lung cancer cell adhesion to extracellular matrix and metastasis by targeting integrin β1 and matrix metalloproteinase2 (MMP2)[J]. PloS one, 2013, 8(8): e70192.
5、 Zhao Y, Yao R, Ouyang L, et al. Three-dimensional printing of Hela cells for cervical tumor model in vitro[J]. Biofabrication, 2014, 6(3): 035001.
6、 Wu Y, Wang Y P, Guo P, et al. A lipoxin A4 analog ameliorates blood–brain barrier dysfunction and reduces MMP-9 expression in a rat model of focal cerebral ischemia–reperfusion injury[J]. Journal of Molecular Neuroscience, 2012, 46(3): 483-491.
7、 Li J, Fan R, Zhao S, et al. Reactive oxygen species released from hypoxic hepatocytes regulates MMP-2 expression in hepatic stellate cells[J]. International journal of molecular sciences, 2011, 12(4): 2434-2447.
8、 Lin Y, Chang G, Wang J, et al. NHE1 mediates MDA-MB-231 cells invasion through the regulation of MT1-MMP[J]. Experimental cell research, 2011, 317(14): 2031-2040.
9、 Zhang Y, Huang L, Wang C, et al. Phenanthrene exposure produces cardiac defects during embryo development of zebrafish (Danio rerio) through activation of MMP-9[J]. Chemosphere, 2013, 93(6): 1168-1175.
10、 Lei F Y, Zhou T B, Qin Y H, et al. Potential signal pathway of all-trans retinoic acid for MMP-2 and MMP-9 expression in injury podocyte induced by adriamycin[J]. Journal of Receptors and Signal Transduction, 2014, 34(5): 378-385.
11、 Deng L, Li G, Li R, et al. Rho-kinase inhibitor, fasudil, suppresses glioblastoma cell line progression in vitro and in vivo[J]. Cancer biology & therapy, 2010, 9(11): 875-884.
12、 Ma S, Yang D, Wang K, et al. Cryptotanshinone attenuates isoprenaline-induced cardiac fibrosis in mice associated with upregulation and activation of matrix metalloproteinase-2[J]. Molecular medicine reports, 2012, 6(1): 145-150.
13、 Zhu G, Kang L, Wei Q, et al. Expression and regulation of MMP1, MMP3, and MMP9 in the chicken ovary in response to gonadotropins, sex hormones, and TGFB1[J]. Biology of reproduction, 2014: biolreprod. 113.114249.
蛋白研究:蛋白提取、蛋白定量、Western Blot…核酸研究:核酸提取、PCR、核酸电泳… 抗体:单克隆抗体、多克隆抗体、酶标抗体… 细胞生物学:转染试剂、生化测定、细胞培养…
联系方式
通信地址:北京市昌平区振兴路36号2号楼3层330
客服电话:010-60781808 010-62027915
公司邮箱:sale@applygen.com
邮政编码:102299
版权所有 北京普利莱基因技术有限公司 Applygen Technologies Inc.
京ICP备05011537号-1